characterization of leishmania isolated from kala- azar infected patients in kermanshah using pcr

Development of a species-specific PCR assay for detection of Leishmania donovani in clinical samples from patients with kala-azar and post-kala-azar dermal leishmaniasis.

We have developed a PCR assay that is capable of amplifying kinetoplast DNA (kDNA) of Leishmania donovani in a species-specific manner among Old World leishmanias. With Indian strains and isolates of L. donovani the assay was sensitive enough to detect kDNA in an amount equivalent to a single parasite or less. The extreme sensitivity of the assay was reflected in its ability to detect parasite ...

Mitotic cycle of the kala-azar parasite, Leishmania donovani.

identification of species of leishmania isolated from patients with cutaneous leishmaniasis in kermanshah; using rapd-pcr technique

annually many numbers of pationts with cutaneous leishmaniasis (cl) have been reported in kermanshah province- iran. the study aimed to identify species of leishmania isolated from patients with ct in kermanshah. seven isolates of leishmania obtained from patients with cl, without any travelling to other provinces, were cultured in nnn medium. after mass production of leptomonads in rpmi 1640 m...

Nested PCR assay for detection of Leishmania donovani in slit aspirates from post-kala-azar dermal Leishmaniasis Lesions.

A nested PCR assay to detect parasite DNA in slit aspirates from skin lesions of patients with post-kala-azar dermal lesihmaniasis (PKDL) is described. PCR results were positive in 27 of 29 (93%) samples by nested PCR assay, while only 20 of 29 (69%) were positive in a primary PCR assay. The nested PCR assay allowed reliable diagnosis of PKDL in a noninvasive manner.

Kala Azar

To the Editor, "Indian Medical Gazette." Sir,?In the communication from Surgn.-Capt. P. W. O'Gorman in your issue for November regarding hda azar I see no mention of the fact of his having made use of the microscope in his search for anchylostomata. If, instead of straining the motion and examining them microscopically, he examines them microscopically according to the directions given by Sonsi...

Conflict and kala-azar: determinants of adverse outcomes of kala-azar among patients in southern Sudan.

We analyzed data obtained from 3365 patients with kala-azar (KA) or post-KA dermal leishmaniasis (PKDL) treated by Medecins Sans Frontieres-Holland in south Sudan from October 1998-May 2002. Patients were malnourished (median body mass index [BMI], 15.5; median weight for height [WFH], 75.5%) and anemic (median hemoglobin (Hb) level, 8.5 g/dL). The proportion of patients with primary KA who wer...